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A comparative “in-vitro anti-inflammatory activity” study of aerial parts of plant hamelia patens

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Author: 
Shweta Singh and Mrs. Manju Vyas Singh
Abstract: 

Background: Inflammation is a complex biological vascular tissues response to harmful stimuli which may including pathogens, irritants or damaged cells. Natural therapy is a key to overcome limitations of present NSAIDs used to treat inflammatory disorder and related symptoms. The plant Hamelia patens belongs to family Rubiaceae, which is rich in active phytochemicals like flavonoids and alkaloids. This Plant has been traditionally used for its beneficial effect in the treatment of various inflammatory disorder and related symptoms. Aim: In the present communication in-vitro anti-inflammatory activity of aerial parts (leaf and stem) of plant Hamelia patens was assessed using different in-vitro anti-inflammatory activity assays. Methods: The anti-inflammatory activity of different extracts i.e methanolic and aqueous extracts of leaf and stem of plant Hamelia patens was assessed using following three methods- membrane stabilization method (HRBC), protein (trypsin) inhibitory method and protein (albumin) denaturation method. Results: Anti-inflammatory activity by HRBC membrane stabilization method was found to be best in methanolic stem extract with IC50 value of 508.28 µg/ml and least in methanolic leaf extract with IC50 value of 916.12µg/ml, anti-inflammatory activity by protein inhibitory method and protein albumin method was found to be highest in aqueous stem extract with IC50value of 259.69 and 2.86µg/ml respectively while least IC50 value was observed in aqueous leaf extract i.e. 1153.29 µg/ml and 9.81 µg/ml by protein inhibitory method and protein albumin method respectively. Conclusion: The result of the present study support the anti-inflammatory activity of plant Hamelia patens. Moreover, the potential anti- inflammatory effect of plants could be enhanced by extracting with methanol instead of water. Although plant proves its role in inflammation and can be used to reduce inflammatory injury and tissue damage but further detailed investigation need to be undertaken by following in-vivo study and then proper isolation and characterization techniques to identify responsible chemical constituent for anti-inflammatory activity.

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